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Amniotic tissue fdating


Amniotic membrane AM can promote proper epithelialization with suppression of excessive fibrosis by creating a supportive milieu for regeneration of chronic ulcer bed. The objective of this study is to investigate whether AM scaffold can modulate the Amniotic tissue fdating of a wound by promoting tissue reconstruction rather than promoting scar tissue formation. AM was obtained and prepared and then applied to patients with chronic leg ulcers who were randomly divided into two different groups.

Group I Amniotic tissue fdating group included eleven patients in whom ulcers were treated with conventional wound dressings that were changed daily for 8 weeks. Group II study group included 14 patients in whom the AM was placed in contact with the ulcer and held in place with a secondary dressing, which was changed daily. Follow-up was done to detect healing rate and detection of ulcer size, assessment of pain, and to take ulcer images days 0, 7, 14, 21, 30, 45, and In group I, all ulcers showed no reduction in their size, and ulcer floor remained the same.

Healthy granulations were present in two ulcers There was no improvement of pain level in the eleven ulcers. In group II, complete healing of 14 ulcers occurred in 14—60 days with a mean of Healthy granulations were present in 13 ulcers "Amniotic tissue fdating" healing rate was faster in ulcers of mild severity 1. AM graft can be of value in wound healing.

Further studies are needed to confirm these findings. Amniotic membrane AM is an attractive method of grafting for wounds as it has unique properties, including anti-inflammatory effects, bacteriostatic, wound protection, decreased scarring, and pain reduction properties, as well as epithelialization initialization capacities. Furthermore, AM is widely available and less costly than other bioengineered skin substitutes.

Human AM was used for 2, ophthalmologic reconstructions in Germany The anti-inflammatory property of AM seems to be a result of production of anti-inflammatory proteins and reduction of expression of transforming growth factor B and pro-inflammatory cytokines, such as interleukin Loeffelbein et al 10 demonstrated accelerated formation of basement membrane in wounds treated with AM that might be due to the release of growth factors.

One of the most important properties of AM as a skin substitute is "Amniotic tissue fdating" relieving which may be due to diminished inflammation, better hydration of wound bed, Amniotic tissue fdating protection of exposed nerve endings.

Mermet et al 14 put an AM graft for 15 chronic leg ulcers and healing occurred in all patients. Pesteil et al Amniotic tissue fdating used cryopreserved AM in eight patients with resistant vascular ulcers.

Tolerance to the graft was excellent with healing of six out of eight patients with significant improved pain. Litwiniuk et al 17 suggested the potential role of matrix metalloproteinase inhibitors present in radiation-sterilized amnion dressing in healing of 23 out of 25 patients with chronic venous ulcers. Sheikh et al 18 used dehydrated amnion to provoke healing of chronic wounds in four patients and healed wounds did not recur on long-term follow-up.

A similar study was done Amniotic tissue fdating Zelen et al 19 who used dehydrated AM in diabetic foot ulcers with complete healing of 37 out of 40 ulcers. With respect to the low cost, wide availability, and easy preparation, AM can be an ideal graft for chronic refractory ulcers.

This was an experimental, comparative, and randomized clinical trial. This study was performed to test a technique for the treatment of chronic nonhealing wounds using AM to express its effect on the rate of healing of such nonhealing ulcers. Each patient signed an informed consent after accepting to be enrolled in the study. Ethical aspects whether substantial or procedural have been implicated in this study and approval was obtained from the Faculty of Medicine, Ethical Committee of Cairo University Patients were then randomly divided into two different groups.

Group I the control group included eleven patients with eleven chronic leg ulcers in whom ulcers were treated with conventional wound dressings that were changed daily for 8 weeks. Group II the study group included 14 patients with 14 chronic leg ulcers.


Amniotic tissue fdating AM was placed in contact with the ulcer and held in place with a secondary dressing, which was changed daily. Inclusion criteria were presence of leg ulcers for more than 3 months with no improvement despite standard treatment and age between 26 and 43 years.

Exclusion criteria were ulcers with ongoing active infection and presence of diabetes.

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